注意：该价格为冻干粉价格,平板加200元,斜面加100元,菌液加150元
产品中文名称：新型隐球酵母 H99
产品英文名称：Cryptococcus neoformans var. grubii
参考用途：生物医学研究和发展材料新兴传染病研究差异基因表达研究中的阴性对照产生漆酶
形态特性：菌落直径为1-2mm，圆形，边缘整齐，不透明，正面乳白色，中间凸起，表面光滑，质地湿润，G＋（蓝紫色），球菌，纯度：纯
培养基：酵母浸粉：3.0，麦芽提取物：3.0，葡萄糖：10.0，酪蛋白胨：5.0，琼脂：20.0，pH：6.2±0.2（25℃）
培养条件：30℃,24-48h,好氧
培养方法：①准备上述平板 1-2 块；②安全柜中打开，用酒精灯灼烧顶部，后迅速滴上无菌水使之破裂，随后用镊子将其敲碎；③吸取 0.5mL 无菌水打入冻干管，充分溶解菌粉后，分别打入两块平板，每块平板打入约200μL，涂布均匀；④将平板置于上述培养条件下培养，菌种长出即可使用。
存储形式：2-8℃
提供形式：冻干粉；斜面；菌液；平板
备注：‖Antigenic Properties│Serotype A RefWilliamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180 1570-1572, 1998. PubMed 9515929, RefFranzot SP, et al. Cryptococcus neoformans var. grubii separate varietal status for Cryptococcus neoformans serotype A isolates. J. Clin. Microbiol. 37 838-840, 1999. PubMed 9986871‖Genome Sequenced Strain│Yes‖Mating Type│Mating type alpha (Peter R Williamson, personal communication)‖Sequenced Data│18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. AAGGATCAGTAGAGAATATTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAG D1D2 region of the 28S ribosomal RNA gene. CATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGGGGAATGTAGCACTCTTCGGAGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC‖Cross References│Nucleotide (GenBank) AF254790 Filobasidiella neoformans var. neoformans vacuolar (H+)-ATPase
Nucleotide (GenBank) AF098972 phosphatidylinositol 3-kinase TOR1 (TOR1), complete coding sequence
Nucleotide (GenBank) AF097888 macrolide-binding protein FKBP12 (FRR1) gene, complete coding sequence
Nucleotide (GenBank) AF097889 macrolide-binding protein FKBP12 (FRR1) mRNA, complete coding sequence
Nucleotide (GenBank) AACO02000000 Cryptococcus neoformans var. grubii H99, whole genome shotgun sequencing project.‖描述│Biomedical Research and Development Material
Emerging infectious disease research
Negative control in differential gene expression studies Refdel Poeta M, et al. Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. Infect. Immun. 67: 1812-1820, 1999. PubMed: 10085022
produces laccase RefWilliamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929;Multigene phylogeny and phenotypic characterization. RefFindley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot. Cell 8: 353-361, 2009. Facultative intracellular pathogen RefFeldmesser M, et al. Cryptococcus neoformans is a facultative intracellular pathogen in murine pulmonary infection. Infect. Immun. 68: 4225-4237, 2000. PubMed: 10858240
Genome sequencing strain (Broad Institute, USA). RefHeitman J, et al. The Cryptococcus neoformans genome sequencing project. Mycopathologia 148: 1-7, 1999.