168425-64-7 | COMPOUND 401
英文名称
COMPOUND 401
分子式
C16H15N3O2
分子量
281.31
结构式
中文别名
英文别名
| 属性 | 值 |
|---|---|
| 密度 | 1.36±0.1 g/cm3 (20 ºC 760 Torr) |
| 形态 | 粉末晶体 |
| 沸点 | 456.9±55.0 °C(Predicted) |
| 熔点 | 184-186℃ (ethanol ) |
| 颜色 | 白色至浅黄色 |
| 溶解度 | DMSO : 6 mg/mL (21.33 mM; Need ultrasonic and warming) |
| 储存条件 | Store at RT |
| CAS 数据库 | 168425-64-7 |
| 酸度系数(pKa) | 1.01±0.20(Predicted) |
| 属性 | 值 |
|---|---|
| 海关编码 | 2934.99.4400 |
| 标题 | 内容 |
|---|---|
| 靶点 | Target Value DNA-PK (Cell-free assay) 0.28 μM mTOR (Cell-free assay) 5.3 μM |
| 体外研究 | Compound 401 is a potent inhibitor of DNA-PK (IC 50 =0.28 μM). Compound 401 is reported to be a poor inhibitor of PI3K, ATM, and ATR in vitro, but it is active against mTOR. Compound 401 shows activity against mTOR (IC 50 =5.3 μM) but not p110α/p85α PI3K (IC 50 >100 μM). Treatment of cells with Compound 401 blocks the phosphorylation of sites modified by mTOR-Raptor and mTOR-Rictor complexes (ribosomal protein S6 kinase 1 Thr 389 and Akt Ser 473 , respectively). By contrast, there is no direct inhibition of Akt Thr 308 phosphorylation, which is dependent on PI3K. Similar effects are also observed in cells that lack DNA-PK. Compound 401 inhibits immunoprecipitated epitope-tagged mTOR or endogenous mTOR in Raptor immunoprecipitates. In both cases, inhibition of 67% or 78% is obtained at 5 μM or 10 μM Compound 401, respectively. By contrast, dose response curves show that the p110α/p85α or p110β/p85α PI3K complexes are poorly inhibited by Compound 401 at these concentrations. The proliferation of TSC1 -/- fibroblasts is inhibited in the presence of Compound 401, but TSC1 +/+ cells are resistant. |
| 生物活性 | Compound 401是DNA-PK和mTOR抑制剂（IC50分别为0.28 μM和5.3 μM）。它对p110α/p85α PI3K没有明显抑制作用，在COS7细胞中抑制S6激酶在Thr389位的磷酸化和Akt在Ser473位的磷酸化。 |