人宫颈癌细胞 H1HeLa
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人宫颈癌细胞 HelaChangLiver
BNCC339985 | 冻存管；T25培养瓶
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人宫颈癌细胞 HeLaS3
BNCC359860 | 冻存管；T25培养瓶
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人子宫颈癌细胞 HT-3
BNCC234836 | 冻存管；T25培养瓶
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人子宫颈鳞状细胞癌细胞 SiHa
BNCC337881 | 冻存管；T25培养瓶
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人宫颈癌细胞基因组DNA Genomic DNA from Hela
BNCC378314 | 冻干粉
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人宫颈癌细胞（荧光素酶标记） HeLa-LUC
BNCC378559 | 冻存管；T25培养瓶
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人子宫颈表皮癌细胞 ME-180
BNCC339303 | 冻存管；T25培养瓶
产品中文名称：人宫颈癌细胞
产品英文名称：H1HeLa
参考用途：The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al.
This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.
形态特性：上皮细胞样，多角形，边缘不规则，单层贴壁生长
培养基：90%L-15+10%FBS
培养条件：100%空气；
培养方法：复苏步骤：①冻存管从液氮或-80℃冰箱中取出放入PE手套中，迅速没入37℃水浴锅，摇晃冻存管加速溶解，以1min内全部溶解为宜；②在超净台中将溶解好的细胞液加入到装有9mL完全培养基的离心管内，1000-1200rpm离心5min，弃去上清液，用1-2mL完全培养基重悬细胞。③将细胞悬液加入到含有5-6mL完全培养基的T25瓶中，放入培养箱培养。
细胞传代：①将旧培养液吸除，PBS清洗两遍后，加入1-2mL胰酶（0.25%Trypsin+0.02%EDTA）；②镜下观察消化情况，在细胞边缘缩小，贴壁松动时（可用吸管吸起些许胰酶轻轻吹打细胞层某处，肉眼可见细胞层脱落，即消化完成，否则继续消化），直接吸掉胰酶，加入5-6mL完全培养基，轻轻吹打细胞层，把细胞层吹落，吹散。③将细胞悬液按1:2比例分到新的T25瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养。；④注意培养基pH值变化和细胞密度，定期换液（每周2-3次），待细胞密度达到80%-90%时，重复传代操作或者冻存。
存储形式：液氮
提供形式：冻存管；T25培养瓶
备注：‖Age│31 years‖Gender│female‖Ethnicity│Black‖Derivation│The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).‖Clinical Data│31 years
Black
female‖HeLa Markers│Y‖Genes Expressed│keratin‖Cellular Products│keratin‖STR Profile│Amelogenin X
CSF1PO 9,10
D13S317 12,13.3
D16S539 9,10
D5S818 11,12
D7S820 8,12
THO1 7
TPOX 8,12
vWA 16,18‖Isoenzymes│G6PD, A‖描述│The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).
This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.
The line is usually carried as monolayer cultures in Leibovitz's L-15 medium, but can be readily adapted to other media and to suspension culture (in media without Ca++).
The H-HeLa line was cured of a mycoplasma contamination by W.M. Lee in Rueckert's laboratory and the cured line was given the designation H1-HeLa.
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