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嗜热脂肪地芽孢杆菌 Geobacillus stearothermophilus (Donk) Nazina et al.
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嗜热脂肪地芽孢杆菌 Geobacillus stearothermophilus (Donk) Nazina et al.

BNCC298315
¥ 1500.0
¥ 1500.0
冻干粉;斜面;菌液;平板
2029-12-31
北纳生物/BNCC
见详情
+
≥10
嗜热脂肪地芽孢杆菌介绍:

注意:该价格为冻干粉价格,平板加200元,斜面加100元,菌液加150元
产品中文名称:嗜热脂肪地芽孢杆菌
产品英文名称:Geobacillus stearothermophilus (Donk) Nazina et al.
参考用途:生产限制性内切核酸酶 BstPI < br > 蒸汽灭菌控制 < br > 无菌保证 < br > 药品和个人护理
形态特性:菌落直径为1-2mm,圆形,边缘不规则,不透明,正面灰白色,浅色,形态扁平,表面粗糙,表面灰暗,质地干燥,易挑起,不产色素,G+(蓝紫色),杆菌,纯度:纯
培养基:蛋白胨:10.0g,牛肉粉:3.0g,氯化钠:5.0g,琼脂:15.0g,pH值:7.3±0.1(25℃)
培养条件:55°C ;18-24h;好氧;
培养方法:①准备1支含有5~10mL液体培养基的试管和2个平板;②安全柜中打开,用酒精灯灼烧顶部,后迅速滴上无菌水使之破裂,随后用镊子将其敲碎;③吸取0.5mL液体培养基打入冻干管,充分溶解后重新打回液体试管中,混匀;④吸取0.2mL菌悬液打入平板中,涂布均匀,重复两次获得两个平板;⑤将液体试管和平板全部置于上述培养条件下培养,菌种长出即可使用。
存储形式:2-8℃
提供形式:冻干粉;斜面;菌液;平板
备注:‖Cross References│Nucleotide (GenBank) U34874 Bacillus stearothermophilus levansucrase (surB) gene, complete cds.
Nucleotide (GenBank) X93600 B.stearothermophilus aspC gene.
Nucleotide (GenBank) M57457 B.stearothermophilus alpha amylase gene, complete cds.
Nucleotide (GenBank) U34875 Bacillus stearothermophilus levanase (surC) gene, complete cds.
Nucleotide (GenBank) M63307 Bacillus stearothermophilus histidine utilization operon (hut) gene sequence.
Nucleotide (GenBank) M26646 B.stearothermophilus manganese superoxide dismutase (MnSOD) gene, complete cds.
Nucleotide (GenBank) AF055578 Bacillus stearothermophilus surface layer protein precursor (sbsC) gene, complete cds.
Nucleotide (GenBank) AF139535 Bacillus stearothermophilus trp RNA-binding attenuation protein (mtrB) gene, complete cds.
Nucleotide (GenBank) AF162268 Bacillus stearothermophilus insertion sequence ISBst12 putative transposase gene, complete cds.
Nucleotide (GenBank) AF139534 Bacillus stearothermophilus chorismate mutase (aroH), anthranilate synthase subunit (trpE), and anthranilate phophoribosyl transferase (trpD) genes, complete cds.

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