产品中文名称：毛霉属脱氧核糖核酸液体室内质控品
产品英文名称：Mucor sp. DNA lab quality control
参考用途：本质控品由河南省工业微生物菌种工程技术研究中心研制，为毛霉属灭活悬液，通过添加稳定剂、数字PCR定值后分装而得，可用于方法建立、工作标准赋值、方法确认与评价、产品质量控制等。
形态特性：小型丝状真菌，在综合 PDA 培养基上菌落明显，初期菌丝白色，后期变灰褐色，培养基背面淡黄色
培养基：马铃薯浸粉：10.0g，葡萄糖：20.0g，KH2PO4：3.0g，MgSO4·7H2O：1.5g，硫胺素：0.008g，琼脂：15.0g，pH：6.0±0.2（25℃）
培养条件：28℃，3-5天，好氧
培养方法：①准备 1 支含有 5~10mL 液体培养基的试管和 2 个平板；②安全柜中打开，用酒精灯灼烧顶部，后迅速滴上无菌水使之破裂，随后用镊子将其敲碎；③吸取 0.5mL 液体培养基打入冻干管，充分溶解后重新打回液体试管中，混匀；④吸取 0.2mL 菌悬液打入平板中，涂布均匀，重复两次获得两个平板；⑤将液体试管和平板全部置于上述培养条件下培养，菌种长出即可使用。
存储形式：-20℃
提供形式：冻存管
备注：‖Antigenic Properties│Serotype A RefWilliamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180 1570-1572, 1998. PubMed 9515929, RefFranzot SP, et al. Cryptococcus neoformans var. grubii separate varietal status for Cryptococcus neoformans serotype A isolates. J. Clin. Microbiol. 37 838-840, 1999. PubMed 9986871‖Genome Sequenced Strain│Yes‖Mating Type│Mating type alpha (Peter R Williamson, personal communication)‖Sequenced Data│18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. AAGGATCAGTAGAGAATATTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAG D1D2 region of the 28S ribosomal RNA gene. CATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGGGGAATGTAGCACTCTTCGGAGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC‖Cross References│Nucleotide (GenBank) AF254790 Filobasidiella neoformans var. neoformans vacuolar (H+)-ATPase
Nucleotide (GenBank) AF098972 phosphatidylinositol 3-kinase TOR1 (TOR1), complete coding sequence
Nucleotide (GenBank) AF097888 macrolide-binding protein FKBP12 (FRR1) gene, complete coding sequence
Nucleotide (GenBank) AF097889 macrolide-binding protein FKBP12 (FRR1) mRNA, complete coding sequence
Nucleotide (GenBank) AACO02000000 Cryptococcus neoformans var. grubii H99, whole genome shotgun sequencing project.‖描述│Biomedical Research and Development Material
Emerging infectious disease research
Negative control in differential gene expression studies Refdel Poeta M, et al. Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. Infect. Immun. 67: 1812-1820, 1999. PubMed: 10085022
produces laccase RefWilliamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929;Multigene phylogeny and phenotypic characterization. RefFindley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot. Cell 8: 353-361, 2009. Facultative intracellular pathogen RefFeldmesser M, et al. Cryptococcus neoformans is a facultative intracellular pathogen in murine pulmonary infection. Infect. Immun. 68: 4225-4237, 2000. PubMed: 10858240
Genome sequencing strain (Broad Institute, USA). RefHeitman J, et al. The Cryptococcus neoformans genome sequencing project. Mycopathologia 148: 1-7, 1999.