产品中文名称：人B淋巴瘤细胞
产品英文名称：Farage
参考用途：/
形态特性：淋巴母细胞样，圆形
培养基：90%RPMI-1640+10%FBS
培养条件：37℃；5%CO2+95%空气；
培养方法：复苏步骤：①冻存管从液氮或-80℃冰箱中取出放入PE手套中，迅速没入37℃水浴锅，摇晃冻存管加速溶解，以1min内全部溶解为宜；②在超净台中将溶解好的细胞液加入到装有9mL完全培养基的离心管内，1000-1200rpm离心3-5min，弃去上清液，用1-2mL完全培养基重悬细胞。③然后将细胞悬液加入到含有6-7mL完全培养基的T25瓶中，放入培养箱培养。培养瓶建议竖着培养。
细胞传代：①离心法：收集细胞，1000rpm离心5min，弃去上清液，补加1-2mL培养液后吹匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。②半量换液法：可选择半量换液方式；请将上清培养基轻轻吸走一半，将剩余留有细胞沉淀的培养基重悬混匀，将细胞悬液按1：2的比例分到新的含8mL培养基的T25瓶中。③注意培养基pH值变化和细胞密度，定期换液（每周2-3次），待细胞密度达到大于2×106个/mL时，重复传代操作或者冻存。
存储形式：液氮
提供形式：冻存管；T25培养瓶
备注：‖Age│adult‖Gender│female‖Ethnicity│White‖Karyotype│trisomy of chromosome 11 RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin’s lymphoma cell lines. Eur. J. Immunol. 63 180-191, 1999. PubMed 10485273‖Derivation│The Farage cell line was adapted to culture in 1990 from a lymph node biopsy of a patient with diffuse large cell non-Hodgkin’s lymphoma (DLCL).‖Clinical Data│adult
female
White‖Antigen Expression│CD10 +/-; CD11a + (LFA-1); CD19 +; CD20 +; CD21 +; CD22 +; CD23 +; CD29 (VLA-4) +; CD38 +; CD39 +; CD40 +; CD44 +; CD54 + (ICAM-1); CD58 + (LFA-3); CD23 -; HLA DR +‖STR Profile│Amelogenin X
CSF1PO 11,12
D13S317 11,13
D16S539 11,12
D5S818 12
D7S820 12
THO1 8,9
TPOX 9
vWA 14,15‖Population Doubling Time│24 to 36 hrs‖Year of Origin│1990‖描述│The cells do not express surface or cytoplasmic immunoglobulin. Exposure to IL-4 augmented the concentrations of CD23, CD54, and CD58 but diminished the expression of CD21, CD22, and CD38. Incubation with IL-4 for 6 to 8 days led to increased expression of CD11a, CD39, CD40, and to disappearance of CD21 and CD38. Exposure of Farage cells to phorbol 12-myristate 13-acetate (PMA) down-regulated CD21 and CD23 expression. They do not express the terminal deoxynucleotydyl transferase gene (TdT), nor the recombination activating genes RAG-1 and RAG-2, known as markers of the pre-B cell stage. These results show that Farage represents a mature B-cell rather than a pre-B cell. The cells are positive for Epstein-Barr virus (EBV).
mature B cell