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表皮葡萄球菌_
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表皮葡萄球菌

BNCC356215 Staphylococcus epidermidis (Winslow and Winslow) Evans {{goodObj.price > 0 ? '¥' + goodObj.price : '咨询'}} ¥{{goodObj.sell_price || 0}} 咨询 冻干粉;斜面;菌液;平板 {{goodObj.date}} 北纳生物/BNCC {{item.norm}} 见详情 {{inventory}}
表皮葡萄球菌介绍:

注意:该价格为冻干粉价格,平板加200元,斜面加100元,菌液加150元
产品中文名称:表皮葡萄球菌
产品英文名称:Staphylococcus epidermidis (Winslow and Winslow) Evans
参考用途:/
形态特性:菌落直径为1-2mm,圆形,边缘整齐,不透明,正面灰白色,中间凸起,表面明亮,表面光滑,质地湿润,G+(蓝紫色),球菌
培养基:蛋白胨:10.0g,牛肉粉:3.0g,氯化钠:5.0g,琼脂:15.0g,pH值:7.3±0.1(25℃)
培养条件:37℃;18-24h;好氧;
培养方法:①准备1支含有5~10mL液体培养基的试管和2个平板;②安全柜中打开,用酒精灯灼烧顶部,后迅速滴上无菌水使之破裂,随后用镊子将其敲碎;③吸取0.5mL液体培养基打入冻干管,充分溶解后重新打回液体试管中,混匀;④吸取0.2mL菌悬液打入平板中,涂布均匀,重复两次获得两个平板;⑤将液体试管和平板全部置于上述培养条件下培养,菌种长出即可使用。
存储形式:2-8℃
提供形式:冻干粉;斜面;菌液;平板
备注:‖Cross References│Nucleotide (GenBank) K02688 S.epidermidis 5S rRNA.
Nucleotide (GenBank) Z48233 S.epidermidis thyF and dfrC genes.
Nucleotide (GenBank) AX109637 Sequence 370 from Patent WO0123604.
Nucleotide (GenBank) AX109878 Sequence 611 from Patent WO0123604.
Nucleotide (GenBank) AX111494 Sequence 2227 from Patent WO0123604.
Nucleotide (GenBank) AX111515 Sequence 2248 from Patent WO0123604.
Nucleotide (GenBank) AX111535 Sequence 2268 from Patent WO0123604.
Nucleotide (GenBank) Z26894 S.epidermidis gene for 16S ribosomal RNA.
Nucleotide (GenBank) AB015166 Staphylococcus epidermidis gene, partial cds.
Nucleotide (GenBank) L37605 Staphylococcus epidermidis 16S ribosomal RNA (16S rRNA) gene.
Nucleotide (GenBank) U02913 Staphylococcus epidermidis ATCC 14990 16S rRNA gene, partial sequence.
Nucleotide (GenBank) D83363 Staphylococcus epidermidis (strain ATCC 14990T) gene for 16S rRNA, partial sequence.
Nucleotide (GenBank) AF298800 Staphylococcus epidermidis translation elongation factor Tu (tuf) gene, partial cds.
Nucleotide (GenBank) AF162863 Staphylococcus epidermidis Pal1 (pal1) gene, partial cds; TagF (tagF) gene, complete cds; and TpgX (tpgX) gene, partial cds.
Nucleotide (GenBank) AF012132 Staphylococcus epidermidis agr system including response regulator (agrA), histidine kinase (agrC), AgrD (agrD), AgrB (agrB) and delta toxin (hld) genes, complete cds.‖描述│ATCC® 14990-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 14990™ frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.  Learn more about ATCC® MinisDeveloped by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go! . Efficacy testing Inhibition testing Media testing Quality control strain Sterility testing Testing antimicrobial handwashing formulations Quality control strain for API products

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相关国家标准解读
标准名称及标准号 GB 4789.10-2016《食品安全国家标准 食品微生物学检验 金黄色葡萄球菌检验》
适用范围 适用于食品及其加工环境中葡萄球菌属(包括表皮葡萄球菌)的定性检测和定量分析,适用于食品中污染菌的监控。
核心检测方法 1. 增菌法:使用7.5%氯化钠肉汤增菌培养;
2. 分离培养:采用Baird-Parker琼脂平板分离;
3. 鉴定:通过凝固酶试验、革兰氏染色及生化反应鉴别。
检出限与定量限 定性检测限为1 CFU/25g(mL);定量检测限为10 CFU/g(mL)。
质控样品要求 需使用阳性质控菌株(如ATCC 25923)验证培养基及试剂有效性,阴性对照需无菌生长。
关键实验步骤 1. 样品均质与增菌;
2. 选择性平板划线培养(35℃±1℃,24-48h);
3. 可疑菌落凝固酶试验及镜检确认。
特别说明 表皮葡萄球菌需与金黄色葡萄球菌区分,需增加甘露醇发酵试验及耐热核酸酶试验。
标准名称及标准号 GB 15982-2012《医院消毒卫生标准》
适用范围 适用于医疗机构环境表面、医疗器械及医务人员手部等表皮葡萄球菌污染的卫生学评价。
核心检测方法 1. 采样:棉拭子法或接触皿法采集表面样本;
2. 培养:血琼脂平板35℃培养48h;
3. 菌落计数及药敏试验(必要时)。
检出限与定量限 接触皿法检测限为1 CFU/皿;棉拭子法定量限为5 CFU/cm²。
质控样品要求 每批次实验需包含空白对照(无菌生理盐水)及阳性对照(表皮葡萄球菌标准菌株)。
关键实验步骤 1. 采样区域标记与标准化处理;
2. 样本运输及保存(4℃,≤4h);
3. 菌落形态学鉴定与计数。
特别说明 需区分表皮葡萄球菌与其他凝固酶阴性葡萄球菌,建议采用自动化鉴定系统辅助。

以上信息仅供参考,请以相应标准的原文为准!