产品中文名称：白假丝酵母定量菌株
产品英文名称：Quantitative strains of candida albicans
参考用途：《消毒技术规范》中医疗器械灭菌效果检测用菌,《GB 4789.15-2016 食品安全国家标准 食品微生物学检验 霉菌和酵母计数》阳性对照菌株，2015版中国药典质控菌株。公共用品用具微生物,GB/T 18204.5-2013 公共场所卫生检验方法 第5部分：集中空调通风系统,GB 4789.15-2016 食品安全国家标准 食品微生物学检验 霉菌和酵母计数,GB 4789.28-2013 食品安全国家标准 食品微生物学检验 培养基和试剂的质量要求；《化妆品技术规范》霉菌和酵母菌检验方法 阳性对照菌株。
形态特性：菌落直径为0.5-1mm，圆形，边缘整齐，不透明，正面白色，中间凸起，表面明亮，表面光滑，质地湿润，易挑起，G＋（蓝紫色），球菌
培养基：酵母浸粉：3.0g，麦芽提取物：3.0g，葡萄糖：10.0g，酪蛋白胨：5.0g，琼脂：20.0g，pH：6.2±0.2（25℃）
培养条件：30℃；24-48h；好氧；
培养方法：①准备1支含有5~10mL液体培养基的试管和2个平板；②安全柜中打开，用酒精灯灼烧顶部，后迅速滴上无菌水使之破裂，随后用镊子将其敲碎；③吸取0.5mL液体培养基打入冻干管，充分溶解后重新打回液体试管中，混匀；④吸取0.2mL菌悬液打入平板中，涂布均匀，重复两次获得两个平板；⑤将液体试管和平板全部置于上述培养条件下培养，菌种长出即可使用。
存储形式：-20℃
提供形式：西林瓶
备注：‖Intended Use │‖Antigenic Properties│Serotype A RefWagner T, et al. pH-dependent denaturation of extracellular aspartic proteinases from Candida species. J. Med. Vet. Mycol. 33 275-278, 1995. PubMed 8531028‖Viability│post-FreezeViability test‖Sequenced Data│18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACATGTGTTTTTCTTTGAAACAAACTTGCTTTGGCGGTGGGCCCAGCCTGCCGCCAGAGGTCTAAACTTACAACCAATTTTTTATCAACTTGTCACACCAGATTATTACTAATAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATATGAATTGCAGATATTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTCCCTCAAACCGCTGGGTTTGGTGTTGAGCAATACGACTTGGGTTTGCTTGAAAGACGGTAGTGGTAAGGCGGGATCGCTTTGACAATGGCTTAGGTCTAACCAAAAACATTGCTTGCGGCGGTAACGTCCACCACGTATATCTTCAAACTTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAGCGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCGTCTTTGGCGTCCGAGTTGTAATTTGAAGAAGGTATCTTTGGGCCCGGCTCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGACCCGGGTCTGTGTAAAGTTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCTTGAGATCAGACTTGGTATTTTGCATGCTGCTCTCTCGGGGGCGGCCGCTGCGGTTTACCGGGCCAGCATCGGTTTGGAGCGGCAGGATAATGGCGGAGGAATGTGGCACGGCTTCTGCTGTGTGTTATAGCCTCTGACGATACTGCCAGCCTAGACCGAGGACTGCGGTTTTTAACCTAGGATGTTGGCATAATGATCTTAA‖Cross References│Nucleotide (GenBank) D86430 Candida albicans gene for CaRho1, complete cds.
Nucleotide (GenBank) AX109674 Sequence 407 from Patent WO0123604.
Nucleotide (GenBank) X56867 C. albicans gene for secretory aspartate proteinase.
Nucleotide (GenBank) AF031229 Candida albicans alternative oxidase (AOX1) gene, complete cds.
Nucleotide (GenBank) U38534 Candida albicans alpha-tubulin (TUB1) gene, complete cds.
Nucleotide (GenBank) L22737 Candida albicans inositol-1-phosphate synthase (IN01) gene, complete cds.
Nucleotide (GenBank) AF031228 Candida albicans D-arabinono-1,4-lactone oxidase (ALO) gene,
Nucleotide (GenBank) U40454 Candida albicans topoisomerase type I (CATOP1) gene, complete cds.
Nucleotide (GenBank) EU266569 ITS including 5.8S rRNA gene‖描述│Assay of amphotericin B fungizone
Assay of antimicrobial preservatives
Assay of haloprogin
Assay of nystatin fungicidin
Media testing
Membrane filter testing
Preparatory test control
Produces D-arabinolactone oxidase
Produces DNA topoisomerase
Food testing
Pharmaceutical and Personal Care
Produces aspartic proteinases aspartyl proteinases
Produces estrogen-binding protein
Produces lanosterol synthase 2,3-oxidosqualene lanosterol cyclase
Produces phenethyl alcohol
Produces polyamine oxidase
Produces tryptophol
Quality control strain
Sterility testing
Testing
Testing fungicides
Produces farnesoic acid, an autoregulatory substance capable of regulating morphological transition
Reference strain for performance testing culture media listed by the ISO TC 34 SC 9 Joint Working Group 5 in the ISO 11133 and by the Working Party on Culture Media of the International Committee on Food Microbiology and Hygiene (ICFMH-WPCM)
This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified.;This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified.
For sterility testing, not more than five passages from the ATCC culture should be used.
Growth and invasiveness in mouse
Steroid interference with antifungal activity
Cell wall hydrophobicity enhances corticosterone incorporation.
Ultraviolet microscopy
Calcification
Morphology and physiology of strain sectors
Use of impedance for preservative efficacy testing
Fungitoxicity of alcohols and fatty acids
Esterase activity
Lipid composition
Effect of antineoplastic drugs