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SW962 [SW 962, SW-962]
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SW962 [SW 962, SW-962]

BNCC312411
SW962 [SW 962, SW-962]
¥ 3000.0
¥ 3000.0
冻存管；T25培养瓶
2029-12-31
北纳生物/BNCC
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≥10
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人阴户磷癌细胞介绍：

产品中文名称：人阴户磷癌细胞
产品英文名称：SW962 [SW 962, SW-962]
参考用途：/
形态特性：上皮细胞样，短梭形，多角形，单层贴壁生长
培养基：90%L-15+10%FBS
培养条件：37℃；100%空气；
培养方法：复苏步骤：①冻存管从液氮或-80℃冰箱中取出放入PE手套中，迅速没入37℃水浴锅，摇晃冻存管加速溶解，以1min内全部溶解为宜；②在超净台中将溶解好的细胞液加入到装有9mL完全培养基的离心管内，1000-1200rpm离心5min，弃去上清液，用1-2mL完全培养基重悬细胞。③将细胞悬液加入到含有5-6mL完全培养基的T25瓶中，放入培养箱培养。
细胞传代：①将旧培养液吸除，PBS清洗两遍后，加入1-2mL胰酶（0.25%Trypsin+0.02%EDTA）；②镜下观察消化情况，在细胞边缘缩小，贴壁松动时（可用吸管吸起些许胰酶轻轻吹打细胞层某处，肉眼可见细胞层脱落，即消化完成，否则继续消化），直接吸掉胰酶，加入5-6mL完全培养基，轻轻吹打细胞层，把细胞层吹落，吹散。③将细胞悬液按1:2比例分到新的T25瓶中，添加适当的完全培养基，把细胞悬液打匀，于培养箱中培养。；④注意培养基pH值变化和细胞密度，定期换液（每周2-3次），待细胞密度达到80%-90%时，重复传代操作或者冻存。
存储形式：液氮
提供形式：冻存管；T25培养瓶
备注：‖Age│64 years‖Gender│female‖Ethnicity│Caucasian‖Karyotype│hypertriploid; modal number = 80; range = 70 to 85. The rate of higher ploidies was 4.3%., At least 15 marker chromosomes were common to most cells. These include t(1p3p), t(1p17p), i(3q), t(8q9q), del(3)(p21), 20q+ and nine others. Of these, i(3q), t(8q9q), del(3)(p21) and a few others were generally paired., An additional segment on 20q+ is long, but its origin is unknown. The total length of 20q+ is as long as that of N1. Normal N3 was absent, and N1 and N18 had only a single copy per cell. Usually, there were three copies of normal X chromosomes.‖Derivation│The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian.‖Clinical Data│The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian.
female
Caucasian
64 years‖Antigen Expression│Blood type A; Rh+‖Genes Expressed│Blood type A; Rh+‖Tumorigenic│Yes‖Effects│Yes, in nude mice(The cells produce tumors consistent with grade I squamous cell carcinoma.)‖Isoenzymes│AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1-2
PGM3, 1‖Passage History│A frozen ampule of the line at passage 14 was transferred to the ATCC in January, 1982.‖描述│The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian.
A frozen ampule of the line at passage 14 was transferred to the ATCC in January, 1982.

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