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人浆细胞白血病 ARH-77
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人浆细胞白血病 ARH-77

BNCC329017
¥ 1200.0
¥ 1200.0
冻存管;T25培养瓶
2029-12-31
北纳生物/BNCC
见详情
+
≥10
人浆细胞白血病介绍:

产品中文名称:人浆细胞白血病
产品英文名称:ARH-77
参考用途:/
形态特性:淋巴母细胞样,圆形,单个细胞,多数聚团
培养基:RPMI-1640完全培养基:90%RPMI-1640+10%FBS
培养条件:37℃;5%CO2+95%空气;
培养方法:复苏步骤:①冻存管从液氮或-80℃冰箱中取出放入PE手套中,迅速没入37℃水浴锅,摇晃冻存管加速溶解,以1min内全部溶解为宜;②在超净台中将溶解好的细胞液加入到装有9mL完全培养基的离心管内,1000-1200rpm离心3-5min,弃去上清液,用1-2mL完全培养基重悬细胞。③然后将细胞悬液加入到含有6-7mL完全培养基的T25瓶中,放入培养箱培养。培养瓶建议竖着培养。
细胞传代:①离心法:收集细胞,1000rpm离心5min,弃去上清液,补加1-2mL培养液后吹匀,将细胞悬液按1:2的比例分到新的含8mL培养基的T25瓶中。②半量换液法:可选择半量换液方式;请将上清培养基轻轻吸走一半,将剩余留有细胞沉淀的培养基重悬混匀,将细胞悬液按1:2的比例分到新的含8mL培养基的T25瓶中。③注意培养基pH值变化和细胞密度,定期换液(每周2-3次),待细胞密度达到大于2×106个/mL时,重复传代操作或者冻存。
存储形式:液氮
提供形式:冻存管;T25培养瓶
备注:‖Age│33 years‖Gender│female‖Ethnicity│Caucasian‖Derivation│The ARH-77 cell line was established from the peripheral blood of a patient suffering from IgG plasma cell leukemia. Although established from cells taken from a patient with a plasma cell leukemia, this line has been shown to be an EBV-transformed B lymphoblastoid cell line.‖Clinical Data│33 years female Caucasian‖Antigen Expression│CD11a +; CD19 +; CD20 +; CD28 +; CD38 -; CD49e +‖STR Profile│D5S818 10,13 D13S317 11,13 D7S820 7,12 D16S539 9,13 vWA 17 THO1 8,9.3 Amelogenin X TPOX 8 CSF1PO 6,10‖Isotype│IgG1; kappa light chain‖描述│The cells are positive for Epstein-Barr nuclear antigen(EBNA +) and Epstein-Barr viral capsid antigen (EBVCA +).
The line has been cured of a previous mycoplasma infection. LICR-LON-HMy2 (HMy2), a fast growing mutant of the ARH-77 cell line (ATCC CRL-1621), was irradiated and selected for class I loss to establish the HMy2.C1R (ATCC CRL-1993) cell line. Subsequently, C1R-neo (ATCC CRL-2369), C1R-sB7 (ATCC CRL-2370), and C1R-B7 (ATCC CRL-2371) were established by transfection of the HMy2.C1R cell line.

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