• 欢迎来到萘析商城
当前位置: 首页 标准物质 菌种 标准菌株 产气克雷伯氏菌
产气克雷伯氏菌_
图片仅供参考,具体产品以实物为准!

产气克雷伯氏菌

BNCC356216 Klebsiella aerogenes Tindall et al. {{goodObj.price > 0 ? '¥' + goodObj.price : '咨询'}} ¥{{goodObj.sell_price || 0}} 咨询 冻干粉;斜面;菌液;平板 {{goodObj.date}} 北纳生物/BNCC {{item.norm}} 见详情 {{inventory}}
产气克雷伯氏菌介绍:

注意:该价格为冻干粉价格,平板加200元,斜面加100元,菌液加150元
产品中文名称:产气克雷伯氏菌
产品英文名称:Klebsiella aerogenes Tindall et al.
参考用途:GB 4789.38-2012大肠埃希氏菌计数
形态特性:菌落直径为1-2mm,圆形,边缘整齐,不透明,正面灰白色,反面灰白色,形态扁平,表面光滑,表面明亮,质地湿润,易挑起,G-(红),杆菌,纯度:纯
培养基:蛋白胨:10.0g,牛肉粉:3.0g,氯化钠:5.0g,琼脂:15.0g,pH值:7.3±0.1(25℃)
培养条件:30°C;18-24h;好氧;
培养方法:①按照培养条件先将菌培养成菌悬液;②测量菌悬液吸光度,按照标准公式计算出菌悬液初始浓度;③初始浓度乘以存活率即为冻干后实际浓度;④根据冻干后实际浓度对菌悬液进行稀释获得目标浓度,然后进行分装、冻干;
存储形式:2-8℃
提供形式:冻干粉;斜面;菌液;平板
备注:‖Cross References│Nucleotide (GenBank) AX110938 Sequence 1671 from Patent WO0123604.
Nucleotide (GenBank) AF052255 Enterobacter aerogenes DNA gyrase A (gyrA) gene, partial cds.
Nucleotide (GenBank) AJ404624 Enterobacter aerogenes marA gene, marC gene (partial), marR gene and marB gene (partial).
Nucleotide (GenBank) AB032468 Enterobacter aerogenes genes for ORF, L-2,4-diaminobutyrate decarboxylase, partial and complete cds.‖描述│ATCC® 13048-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 13048™ frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.  Learn more about ATCC® MinisDeveloped by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go! . Control Culture
Assay of antimicrobial preservatives
Bacterial resistance testing adhesives
Bacterial resistance testing paint
Quality control strain
Reference material
Testing slimicide
Quality control strain for BBL, bioMérieux Vitek, and Difco products
Water testing;This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method D4783-89 where only the taxon is specified.

您正在浏览的产品:产气克雷伯氏菌

手机版:产气克雷伯氏菌

以上信息仅供参考,请以实物批次为准!

本公司销售的所有产品仅供实验科研使用,不用于人体及临床诊断。

标准名称及标准号
食品安全国家标准 食品微生物学检验 克雷伯氏菌检验(GB 4789.38-2021)
适用范围
适用于食品中产气克雷伯氏菌(Klebsiella aerogenes)的定性检测,包括乳制品、肉制品、水产品等样品类型。
核心检测方法
采用选择性培养基(如VRB-MUG琼脂)进行分离培养,结合生化鉴定(吲哚试验、VP试验、柠檬酸盐利用等)和分子生物学方法(PCR或测序)确认目标菌。
检出限与定量限
定性检测的灵敏度为1 CFU/25 g(或mL),定量检测需根据样品稀释度计算,最低报告限值为10 CFU/g。
质控样品要求
每批次实验需同步测试阳性对照(标准菌株ATCC 13048)和阴性对照(大肠埃希氏菌ATCC 25922),平行样数量≥2个,结果偏差应符合标准要求。
关键实验步骤
1. 样品前处理(25 g样品+225 mL缓冲液均质);
2. 选择性增菌(36℃培养18-24 h);
3. 平板划线分离(VRB-MUG琼脂,36℃培养24 h);
4. 典型菌落生化鉴定;
5. 结果判定与报告。
特别说明
需注意与肺炎克雷伯氏菌的鉴别(脲酶试验阴性),实验废弃物需按生物安全二级(BSL-2)要求处理,操作人员需佩戴防护装备。

以上信息仅供参考,请以相应标准的原文为准!