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粪肠球菌_
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粪肠球菌

BNCC290708 Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz {{goodObj.price > 0 ? '¥' + goodObj.price : '咨询'}} ¥{{goodObj.sell_price || 0}} 咨询 冻干粉;斜面;菌液;平板 {{goodObj.date}} 北纳生物/BNCC {{item.norm}} 见详情 {{inventory}}
粪肠球菌介绍:

注意:该价格为冻干粉价格,平板加200元,斜面加100元,菌液加150元
产品中文名称:粪肠球菌
产品英文名称:Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz
参考用途:用于食品中金黄色葡萄球菌的纯培养(GB标准)
形态特性:菌落直径为0.5-1mm,圆形,边缘整齐,不透明,正面乳白色,中间凸起,表面光滑,表面明亮,质地湿润,易挑起,G+(蓝紫色),球菌,纯度:纯
培养基:牛脑浸粉:4.0g,牛心浸粉:4.0g,蛋白胨:5.0g,酪蛋白胨:16.0g,氯化钠:5.0g,葡萄糖:2.0g,磷酸氢二钠:2.5g,琼脂:13.5g,pH:7.4±0.2(25℃)
培养条件:37℃;18-24h;好氧
培养方法:①准备上述平板 1-2 块;②安全柜中打开,用酒精灯灼烧顶部,后迅速滴上无菌水使之破裂,随后用镊子将其敲碎;③吸取 0.5mL 无菌水打入冻干管,充分溶解菌粉后,分别打入两块平板,每块平板打入约200μL,涂布均匀;④将平板置于上述培养条件下培养,菌种长出即可使用。
存储形式:2-8℃
提供形式:冻干粉;斜面;菌液;平板
备注:‖Cross References│Nucleotide (GenBank) AX109558 Sequence 291 from Patent WO0123604.
Nucleotide (GenBank) AX109874 Sequence 607 from Patent WO0123604.‖描述│Food testing
Control strain Ref Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A. Ref Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M26-A.
Evaluation of Mueller-Hinton agar Ref Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.
Media testing Ref Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. Ref Listeria species -- Biochemical Identification Method (Vitek GPI and GNI+). Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 992.19. Ref Water microbiology. Method2: Culture media, diluents and reagents. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.2-1995. Ref Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003. Ref Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003 Ref Microbiology of food and animal feeding stuffs --- Horizontal method for the detection and enumeration of Listeria monocytogenes --- Part 1: Detection method, Annex B [BS 5763-18:1997]. London, UK:British Standards Institution;British Standard BS EN ISO 11290-1:1997. Ref Microbiology of food and animal feeding stuffs --- Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli -- Part 3: Most probable number techniques using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide. London, UK:British Standards Institution;British Standard DD ISO/TS 16649-3:2005. Ref Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.
Quality control Ref Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A.
Quality control strain RefBD Phoenix Gram Positive AST PMIC/ID QC Set. BD Becton Dickinson; Phoenix  Ref MicroScan Dried Gram Positive Panel QC Set. Dade/Siemens. Ref Sensititre™ Automated Gram Positive ID. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems. Ref Sensititre™ MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems. ref
Susceptibility disc testing Ref Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. Ref Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A. Ref Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A.
Susceptibility testing Ref Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8. Ref Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3. Ref Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007 Ref Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M24-A. Ref Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P. RefMedical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-3: 1989 Ref Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-7: 1994, 1994 Ref Medical microbiology: methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents; determination of MICs by agar dilution method. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-6: 1989, 1989 RefMedical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002 Ref Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006.
Quality control strain for API, bioMerieux VITEK, IDS, Micro-Media, MicroScan, and Sensititre products

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本公司销售的所有产品仅供实验科研使用,不用于人体及临床诊断。

匹配国家标准解读
标准名称及标准号 GB/T 5750.12-2023《生活饮用水标准检验方法 微生物指标》(参考DIN 58940-3)
适用范围 适用于生活饮用水及水源水中粪肠球菌的定性及定量检测,涵盖膜过滤法、酶底物法等。
核心检测方法 膜过滤法(MF)为主:水样经滤膜过滤后,接种至选择性培养基(如mEI琼脂),37℃培养24小时,计数典型菌落。
检出限与定量限 检出限为1 CFU/100 mL,定量范围为1-200 CFU/滤膜,超出范围需稀释后复测。
质控样品要求 每批次实验需同步检测阳性对照(ATCC 29212标准菌株)及阴性对照(无菌水),阳性回收率应≥70%。
关键实验步骤 1. 水样过滤(孔径0.45μm);
2. 滤膜贴于mEI琼脂培养;
3. 菌落形态鉴定(蓝色或绿色菌落);
4. 必要时进行革兰氏染色及过氧化氢酶试验验证。
特别说明 若水样含抑菌物质,需在采样后2小时内添加硫代硫酸钠中和;实验环境需符合GB 19489生物安全要求。
标准名称及标准号 GB 4789.38-2021《食品安全国家标准 食品微生物学检验 大肠埃希氏菌计数》(参考BS 5763-18)
适用范围 适用于食品及加工环境中肠球菌属的检测,包括粪肠球菌的分离与计数。
核心检测方法 倾注平板法:样品均质后接种至KF链球菌琼脂,36℃培养48小时,典型粉红色菌落需进一步生化确认。
检出限与定量限 最低检出限为10 CFU/g(液体样品为1 CFU/mL),定量上限为300 CFU/平板。
质控样品要求 每20个样品需设置1个空白对照及阳性对照,阳性菌株为ATCC 19433,允许偏差±0.5 log值。
关键实验步骤 1. 样品预处理(25g+225mL缓冲液);
2. 梯度稀释并倾注培养基;
3. 耐盐试验(6.5% NaCl肉汤);
4. 胆汁七叶苷水解试验确认。
特别说明 冷冻样品需在45℃以下解冻≤15分钟;检测结果报告需注明是否包含受损菌体。

以上信息仅供参考,请以相应标准的原文为准!