注意:该价格为冻干粉价格,平板加200元,斜面加100元,菌液加150元 产品中文名称:大肠埃希氏菌 产品英文名称:Escherichia coli (Migula) Castellani and Chalmers 参考用途:/ 形态特性:菌落直径为1-2mm,圆形,边缘整齐,不透明,正面黄色,颜色较浅,中间凸起,表面光滑,质地湿润,易挑起,G-(红),杆菌 培养基:蛋白胨:10.0g,牛肉粉:3.0g,氯化钠:5.0g,琼脂:15.0g,pH值:7.3±0.1(25℃) 培养条件:37℃;18-24h;好氧; 培养方法:①准备2个平板;②安全柜中打开,用酒精灯灼烧顶部,后迅速滴上无菌水使之破裂,随后用镊子将其敲碎;③吸取0.5mL无菌水打入冻干管,充分溶解,混匀;④吸取0.2mL菌悬液打入平板中,涂布均匀,重复两次获得两个平板;⑤将平板全部置于上述培养条件下培养,菌种长出即可使用。 存储形式:2-8℃ 提供形式:冻干粉;斜面;菌液;平板 备注:‖Antigenic Properties│Serotype O18acK1H7 RefBortolussi R, Ferrieri P. Protection against Escherichia coli K1 infection in newborn rats by antibody to K1 capsular polysaccharide antigen. Infect. Immun. 28 111-117, 1980. PubMed 6769816, RefBortolussi R, et al. Capsular K1 polysaccharide of Escherichia coli relationship to virulence in newborn rats and resistance to phagocytosis. Infect. Immun. 25 293-298, 1979. PubMed 383617, RefBortolussi R, et al. Influence of growth temperature of Escherichia coli on K1 capsular antigen production and resistance to opsonization. Infect. Immun. 39 1136-1141, 1983. PubMed 6341228‖Antigenic Properties│Serotype O18acK1H7 RefBortolussi R, Ferrieri P. Protection against Escherichia coli K1 infection in newborn rats by antibody to K1 capsular polysaccharide antigen. Infect. Immun. 28 111-117, 1980. PubMed 6769816, RefBortolussi R, et al. Capsular K1 polysaccharide of Escherichia coli relationship to virulence in newborn rats and resistance to phagocytosis. Infect. Immun. 25 293-298, 1979. PubMed 383617, RefBortolussi R, et al. Influence of growth temperature of Escherichia coli on K1 capsular antigen production and resistance to opsonization. Infect. Immun. 39 1136-1141, 1983. PubMed 6341228‖Year of Origin│1975‖描述│This strain contains the K1 capsular polysaccharide antigen linked to meningitis in newborn infants. It is resistant to opsonization and phagocytosis.
注意:该价格为冻干粉价格,平板加200元,斜面加100元,菌液加150元
产品中文名称:大肠埃希氏菌
产品英文名称:Escherichia coli (Migula) Castellani and Chalmers
参考用途:/
形态特性:菌落直径为1-2mm,圆形,边缘整齐,不透明,正面黄色,颜色较浅,中间凸起,表面光滑,质地湿润,易挑起,G-(红),杆菌
培养基:蛋白胨:10.0g,牛肉粉:3.0g,氯化钠:5.0g,琼脂:15.0g,pH值:7.3±0.1(25℃)
培养条件:37℃;18-24h;好氧;
培养方法:①准备2个平板;②安全柜中打开,用酒精灯灼烧顶部,后迅速滴上无菌水使之破裂,随后用镊子将其敲碎;③吸取0.5mL无菌水打入冻干管,充分溶解,混匀;④吸取0.2mL菌悬液打入平板中,涂布均匀,重复两次获得两个平板;⑤将平板全部置于上述培养条件下培养,菌种长出即可使用。
存储形式:2-8℃
提供形式:冻干粉;斜面;菌液;平板
备注:‖Antigenic Properties│Serotype O18acK1H7 RefBortolussi R, Ferrieri P. Protection against Escherichia coli K1 infection in newborn rats by antibody to K1 capsular polysaccharide antigen. Infect. Immun. 28 111-117, 1980. PubMed 6769816, RefBortolussi R, et al. Capsular K1 polysaccharide of Escherichia coli relationship to virulence in newborn rats and resistance to phagocytosis. Infect. Immun. 25 293-298, 1979. PubMed 383617, RefBortolussi R, et al. Influence of growth temperature of Escherichia coli on K1 capsular antigen production and resistance to opsonization. Infect. Immun. 39 1136-1141, 1983. PubMed 6341228‖Antigenic Properties│Serotype O18acK1H7 RefBortolussi R, Ferrieri P. Protection against Escherichia coli K1 infection in newborn rats by antibody to K1 capsular polysaccharide antigen. Infect. Immun. 28 111-117, 1980. PubMed 6769816, RefBortolussi R, et al. Capsular K1 polysaccharide of Escherichia coli relationship to virulence in newborn rats and resistance to phagocytosis. Infect. Immun. 25 293-298, 1979. PubMed 383617, RefBortolussi R, et al. Influence of growth temperature of Escherichia coli on K1 capsular antigen production and resistance to opsonization. Infect. Immun. 39 1136-1141, 1983. PubMed 6341228‖Year of Origin│1975‖描述│This strain contains the K1 capsular polysaccharide antigen linked to meningitis in newborn infants. It is resistant to opsonization and phagocytosis.
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2. 选择合适稀释度接种至培养基;
3. 36℃±1℃培养18-24小时;
4. 典型菌落计数及生化确认。
以上信息仅供参考,请以相应标准的原文为准!