改良Letheen琼脂基础（颗粒）/用于测定季铵化合物的细菌活性_培养基_菌种_标准物质_萘析商城

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改良Letheen琼脂基础（颗粒）/用于测定季铵化合物的细菌活性

HBKP8603-3-250

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250g

hopebio

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改良Letheen琼脂基础（颗粒）介绍：

Modified Letheen Agar Base is used for the determination of bacterial activity of quaternary ammonium compounds.

Approximate Formula:

Ingredients	gm/liter
Peptone from Meat	10.0
Peptone from Casein	10.0
Meat Extract	3.0
Yeast Extract	2.0
Sodium Chlorid	5.0
D(+) Glucose	1.0
Lecithin	1.0
Sodium Bisulfite	0.1
Agar	20.0
Final pH7.2±0.2 at 25℃

*Adjusted and/or supplemented as required to meet performance criteria.

Directions:

Suspend 52.1g of the medium and 7 ml of Tween-80 in one liter of distilled water or deionized water. Mix well. Heat and boil for 1 minute with frequent agitation to completely dissolve the powder. Sterilize in an autoclave at 121℃(15 lbs.) for 15 minutes. Pour into petri dishes.

Principle and Interpretation:

Peptone, meat extract and yeast extract provide nitrogen source for the growth of microorganisms, vitamin and growth factor. Sodium chloride provides a suitable osmotic environment. Glucose is a fermentable sugar. Lecithin can neutralize quaternary ammonium salt, Tween-80 can neutralize phenol, hexachlorophenol, formalin, the combination of which can neutralize ethanol. Sodium bisulfite partially neutralize the preservative systems commonly found in cosmetics. Agar is the solidifying agent of the medium.

Appearance:

Dehydrated medium is a free-flowing yellowish granule. The prepared medium is a kind of yellowish transparent gel.

Precautions:

This medium is for laboratory use only. Dried medium which is past shelf life, caking or color variation cannot be used.

Storage conditions and Shelf life:

Modified Letheen Agar Base must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.

Quality control:

Prepare the culture medium as per label directions. Inoculate and incubate at 36±1℃ for 24 hours.

Microorganism	Strains Number	Inoculum (CFU)	Growth	Recovery
Staphylococcus aureus	ATCC 6538	10-100	Luxuriant	≥70%
Pseudomonas aeruginosa	ATCC 9027	10-100	Luxuriant	≥70%
Escherichia coli	ATCC 25922	10-100	Luxuriant	≥70%
Salmonella typhimurium	ATCC 14028	10-100	Luxuriant	≥70%

References:1. FDA Bacteriological Analytical Manual (BAM), 8th ed. (1995), chapter 23:Microbiological Methods for Cosmetics, Letheen Agar (modified) = M 78,etheen Broth (modified) = M 79.

改良Letheen琼脂基础（颗粒）相关产品：

产品货号	产品名称	产品规格	产品说明及用途
HB9241	改良Eugon LT肉汤（2022版ISO 11930标准）Modified Eugon LT Broth	250g	用于在含或不含防腐剂的化妆品中需氧菌增菌培养
HB5181	SCDLP液体培养基Soya Casein Digest Lecithin Polysorbate Broth	250g	用于化妆品样品制备前增菌培养
HB5182	卵磷脂吐温80营养琼脂【别名：营养琼脂(含中和剂)】Lecithin Tween 80 Nutrient Agar	250g	用于化妆品细菌总数测定
HB5183	乙酰胺琼脂Acetamide Agar	250g	用于绿脓杆菌的选择性分离培养（GB标准）
HB5184	十六烷三甲基溴化铵琼脂Cetrimide Agar	250g	用于绿脓杆菌的选择性分离培养（GB标准）
HB8468	甘露醇发酵培养基Mannitol Fermention Medium	250g	用于细菌甘露醇发酵试验（GB标准）
HB5187-5	绿脓菌素测定用假单胞菌琼脂培养基（PAP）Pseudomonas Agar Medium for Detection of Pyocyanin	250g	用于绿脓杆菌的绿脓菌素测定试验
HB5189	TTC卵磷脂-吐温80-营养琼脂TTC Lecithin Tween80 Nutrient Agar	250g	用于化妆品中细菌总数测定
HB5224	HC琼脂基础HC Agar Base	250g	用于化妆品中霉菌总数测定
HB5226	TAT肉汤	250g	用于化妆品和药品中微生物检测
HBPT4116-2	亚碲酸钾卵黄增菌液管（20ml）	20ml*20支/盒	加入到Baird-Parker琼脂基础中作为添加剂使用
HBPP4116-50	亚碲酸钾卵黄增菌液瓶装（50ml）	50ml*20瓶	加入到Baird-Parker琼脂基础中作为添加剂使用
HB5187	绿脓菌素测定培养基（PDP）Pseudomonas Agar Medium for Detection of Pyocyanin	250g	用于绿脓杆菌的绿脓菌素测定试验（GB标准）
HB0235-5	沙氏葡萄糖琼脂(ISO标准)Sabouraud’s Glucose Agar	250g	用于化妆品中白色念球菌分离培养
HB0235-4	沙氏葡萄糖氯霉素琼脂Sabouraud’s Glucose chloramphenicol Agar	250g	用于化妆品中白色念球菌分离培养
HB4119	7.5%氯化钠肉汤7.5% Sodium Chloride Broth	250g	用于金黄色葡萄球菌的增菌培养（GB标准）
HB4116-1	亚碲酸钾卵黄增菌液	5ml*10	每支添加于95mlBaird-Parker琼脂基础或琼脂培养基O中
HB4115	Baird-Parker琼脂基础Baird-Parker Agar Base	250g	用于金黄色葡萄球菌的选择性分离培养（GB、FDA、ISO、SN标准）
HB8604	Letheen肉汤Letheen Broth	250g	用于化妆品中微生物检测
HB8603	Letheen琼脂Letheen Agar	250g	用于化妆品中微生物检测
HB8597-1	改良HC琼脂添加剂	1ml*5	每支添加于200ml改良HC琼脂中
HB8597	改良HC琼脂HC Agar,Modified	250g	用于肠出血性大肠杆菌培养
HB8593	双料乳糖胆盐（含中和剂）培养基Double Lactose Bile Medium	250g	用于化妆品中粪大肠菌群的测定（GB标准）
HB5188	乳糖胆盐培养基Lactose Bile Medium	250g	用于化妆品中粪大肠菌群的测定（GB标准）
HB5220-1	硝酸盐蛋白胨水培养基Nitrate peptone water medium	250g	用于化妆品中铜绿假单胞菌的检测
HB8604-1	MLEB（改良Letheen肉汤）MLEB(Modified Letheen Broth)	250g	用于化妆品中微生物检测
HBPT5181-1	SCDLP液体培养基管（10ml）Soya Casein Digest Lecithin Polysorbate Broth Tube	10ml*20支/盒	用于化妆品的样品制备以及前增菌
HB8535-1	艾格LT100琼脂Eugon LT 100 Agar	250g	用于含或不含防腐剂的化妆用品的菌落总数检验
HB0237-3	虎红（孟加拉红）培养基Rose Bengal Medium	250g	用于霉菌和酵母菌总数的确定（2015版化妆品规范）

以上信息仅供参考，请以实物批次为准！